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TFG Antibody

LS-C409319
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTFG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TFG Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 30kDa/31kDa/43kDa, while the observed MW by Western blot was 55kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 30kDa/31kDa/43kDa, while the observed MW by Western blot was 55kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10342
  • Target name
    TFG
  • Target description
    trafficking from ER to golgi regulator
  • Target synonyms
    HMSNP; protein TFG; SPG57; TF6; TRK-fused; TRK-fused gene protein; TRKT3; TRKT3 oncogene
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRK fused gene/TFG using anti-TRK fused gene/TFG antibody (A02870-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human Hacat whole cell lysates, Lane 8: human T-47D whole cell lysates, Lane 9: rat pancreas tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse small intestine tissue lysates, Lane 12: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRK fused gene/TFG antigen affinity purified polyclonal antibody (Catalog # A02870-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRK fused gene/TFG at approximately 60 kDa. The expected band size for TRK fused gene/TFG is at 43 kDa.
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Lane 1: 293T Cells; Lane 2: Hela Cells; Probed with TRK fused gene (5C1) Monoclonal Antibody (bsm-52676R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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