HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:500 and competitor's antibody (sc-274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
![TFIIB antibody [N1C3] detects TFIIB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TFIIB stained by TFIIB antibody [N1C3] (GTX107327) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107327/GTX107327_40009_20180814_IHC-P_w_23060120_316.webp "TFIIB antibody [N1C3] detects TFIIB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TFIIB stained by TFIIB antibody [N1C3] (GTX107327) diluted at 1:500.")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107327/GTX107327_43117_20180316_WB_w_23060120_818.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1500 and competitor's antibody (sc-274) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107327/GTX107327_40009_20170811_WB_Fraction_competitor_watermark_w_23060120_690.webp "HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1500 and competitor's antibody (sc-274) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![TFIIB antibody [N1C3] detects TFIIB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFIIB stained by TFIIB antibody [N1C3] (GTX107327) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107327/GTX107327_40009_20180523_ICC_IF_w_23060120_842.webp "TFIIB antibody [N1C3] detects TFIIB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TFIIB stained by TFIIB antibody [N1C3] (GTX107327) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10μm.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107327/GTX107327_43117_20180316_WB_B_w_23060120_534.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:500 and competitor's antibody (sc-274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
TFIIB antibody [N1C3]
GTX107327
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGTF2B
Overview
- SupplierGeneTex
- Product NameTFIIB antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.56 mg/ml
- ConjugateUnconjugated
- Gene ID2959
- Target nameGTF2B
- Target descriptiongeneral transcription factor IIB
- Target synonymsTF2B, TFIIB, transcription initiation factor IIB, RNA polymerase II transcription factor IIB, S300-II, general transcription factor TFIIB
- HostRabbit
- IsotypeIgG
- Protein IDQ00403
- Protein NameTranscription initiation factor IIB
- Scientific DescriptionThis gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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