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ProductsBack/TH / Tyrosine Hydroxylase Antibody (N-Terminus)

TH / Tyrosine Hydroxylase Antibody (N-Terminus)

LS-C40043
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTH
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TH / Tyrosine Hydroxylase Antibody (N-Terminus)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in ELISA and Western Blot. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:1000) Suitable for use in ELISA and Western Blot. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7054
  • Target name
    TH
  • Target description
    tyrosine hydroxylase
  • Target synonyms
    DYT14, DYT5b, TYH, tyrosine 3-monooxygenase, dystonia 14, tyrosine 3-hydroxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Tyrosine Hydroxylase/TH using anti-Tyrosine Hydroxylase/TH antibody (PB9449). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosine Hydroxylase/TH antigen affinity purified polyclonal antibody (Catalog # PB9449) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase/TH at approximately 59 kDa. The expected band size for Tyrosine Hydroxylase/TH is at 59 kDa.
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