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WB analysis of various samples using GTX66734 Thrombomodulin antibody. Lane1 : HEK293T cell lysate Lane2 : Raw264.7 cell lysate Lane3 : PC12 cell lysate Dilution : 1:500
WB analysis of various samples using GTX66734 Thrombomodulin antibody. Lane1 : HEK293T cell lysate Lane2 : Raw264.7 cell lysate Lane3 : PC12 cell lysate Dilution : 1:500
WB analysis of various samples using GTX66734 Thrombomodulin antibody. Lane1 : HEK293T cell lysate Lane2 : Raw264.7 cell lysate Lane3 : PC12 cell lysate Dilution : 1:500

Thrombomodulin antibody

GTX66734
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetTHBD
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Overview

  • Supplier
    GeneTex
  • Product Name
    Thrombomodulin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7056
  • Target name
    THBD
  • Target description
    thrombomodulin
  • Target synonyms
    AHUS6; BDCA3; BDCA-3; CD141; CD141 antigen; fetomodulin; THPH12; THRM; thrombomodulin; TM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07204
  • Protein Name
    Thrombomodulin
  • Scientific Description
    The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (Catalog # A01325-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
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