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ProductsBack/Anti-Thrombomodulin [QBEND/40]

Anti-Thrombomodulin [QBEND/40]

AB00975-10.0-BT
Absolute Antibody
ApplicationsElectron Microscopy, Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rabbit
TargetTHBD
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Thrombomodulin [QBEND/40]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Immunomagnetic Dynabeads coated with the endothelial-specific MAb, QBEND-40, provided a rapid and reproducible method for purifying endothelial cells from mixed cell suspensions of first trimester decidual tissues. QBEND-40 was also used in immunohistochemical experiments to charcterise endothelial cells (Drake 1991). Further, QBEND-40 has been used in immunofluorescence experiments with a fluorescent secondary antibody (Massi 2017), and in immunoscanning electron microscopy with secondary antibodies conjugated to gold particles (Wong 2008).
  • Applications
    Electron Microscopy, Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    QBEND/40
  • Gene ID7056
  • Target name
    THBD
  • Target description
    thrombomodulin
  • Target synonyms
    AHUS6, BDCA-3, BDCA3, CD141, THPH12, THRM, TM, thrombomodulin, CD141 antigen, fetomodulin
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP07204
  • Protein Name
    Thrombomodulin
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human, Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (Catalog # A01325-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
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