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Thymidine Kinase antibody [3B3.E11]

GTX20988
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTK1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Thymidine Kinase antibody [3B3.E11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 10 microg/ml. ELISA: 2-5 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3B3.E11
  • Conjugate
    Unconjugated
  • Gene ID7083
  • Target name
    TK1
  • Target description
    thymidine kinase 1
  • Target synonyms
    cytosolic thymidine kinase; epididymis secretory sperm binding protein; thymidine kinase, cytosolic; TK2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04183
  • Protein Name
    Thymidine kinase, cytosolic
  • Scientific Description
    The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TK1 using anti-TK1 antibody (PB9832). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate, Lane 2: SMMC Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TK1 antigen affinity purified polyclonal antibody (Catalog # PB9832) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TK1 at approximately 25 kDa. The expected band size for TK1 is at 25 kDa.
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WB analysis of various sample lysates using GTX33544 Thymidine Kinase 1 antibody. Dilution : 1:1000 Loading : 25microg per lane
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