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ProductsBack/TIF-IA / RRN3 Antibody

TIF-IA / RRN3 Antibody

LS-C813526
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRRN3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TIF-IA / RRN3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54700
  • Target name
    RRN3
  • Target description
    RRN3 homolog, RNA polymerase I transcription factor
  • Target synonyms
    A-270G1.2; RNA polymerase I-specific transcription initiation factor RRN3; RRN3 RNA polymerase I transcription factor homolog; TIFIA; TIF-IA; transcription initiation factor IA; transcription initiation factor TIF-IA
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRN3 using anti-RRN3 antibody (A05439-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRN3 antigen affinity purified polyclonal antibody (Catalog # A05439-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRN3 at approximately 74 kDa. The expected band size for RRN3 is at 74 kDa.
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