IHC-P analysis of human breast adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human colon adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83493/GTX83493_1463_IHC-P_w_23061419_599.webp "IHC-P analysis of human colon adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83493/GTX83493_1464_IHC-P_w_23061419_486.webp "IHC-P analysis of human ovary adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human lung carcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83493/GTX83493_1465_IHC-P_w_23061419_347.webp "IHC-P analysis of human lung carcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human kidney tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83493/GTX83493_1466_IHC-P_w_23061419_651.webp "IHC-P analysis of human kidney tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human liver tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83493/GTX83493_1467_IHC-P_w_23061419_762.webp "IHC-P analysis of human liver tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![WB analysis of HEK293T cells transfected with TIF1 gamma plasmid (Right) or empty vector (Left) for 48 hrs using GTX83493 TIF1 gamma antibody [6B7]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83493/GTX83493_3670_WB_w_23061419_804.webp "WB analysis of HEK293T cells transfected with TIF1 gamma plasmid (Right) or empty vector (Left) for 48 hrs using GTX83493 TIF1 gamma antibody [6B7]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with TIF1 gamma plasmid using GTX83493 TIF1 gamma antibody [6B7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83493/GTX83493_690_ICCIF_w_23061419_737.webp "ICC/IF analysis of COS7 cells transiently transfected with TIF1 gamma plasmid using GTX83493 TIF1 gamma antibody [6B7].")
IHC-P analysis of human breast adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
TIF1 gamma antibody [6B7]
GTX83493
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTRIM33
Overview
- SupplierGeneTex
- Product NameTIF1 gamma antibody [6B7]
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6B7
- Concentration0.85 mg/ml
- ConjugateUnconjugated
- Gene ID51592
- Target nameTRIM33
- Target descriptiontripartite motif containing 33
- Target synonymsE3 ubiquitin-protein ligase TRIM33; ECTO; ectodermin homolog; protein Rfg7; PTC7; RET-fused gene 7 protein; RFG7; RING-type E3 ubiquitin transferase TRIM33; TF1G; TIF1G; TIF1GAMMA; TIF1-gamma; TIFGAMMA; transcriptional intermediary factor 1 gamma
- HostMouse
- IsotypeIgG2b
- Scientific DescriptionActs as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203