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ProductsBack/TIGD1 antibody [C3], C-term

TIGD1 antibody [C3], C-term

GTX107727
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetTIGD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TIGD1 antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID200765
  • Target name
    TIGD1
  • Target description
    tigger transposable element derived 1
  • Target synonyms
    EEYORE; tigger transposable element-derived protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96MW7
  • Protein Name
    Tigger transposable element-derived protein 1
  • Scientific Description
    The protein encoded by this gene belongs to the tigger subfamily of the pogo superfamily of DNA-mediated transposons in humans. These proteins are related to DNA transposons found in fungi and nematodes, and more distantly to the Tc1 and mariner transposases. They are also very similar to the major mammalian centromere protein B. The exact function of this gene is not known. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGD1 antigen affinity purified polyclonal antibody (Catalog # A17411-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIGD1 at approximately 68-70 kDa. The expected band size for TIGD1 is at 67 kDa.
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