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Western Blot analysis of 293 cells using TIGD1 Polyclonal Antibody
Western Blot analysis of 293 cells using TIGD1 Polyclonal Antibody
Western Blot analysis of 293 cells using TIGD1 Polyclonal Antibody

TIGD1 Antibody

CSB-PA008033
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTIGD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TIGD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200765
  • Target name
    TIGD1
  • Target description
    tigger transposable element derived 1
  • Target synonyms
    EEYORE, tigger transposable element-derived protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96MW7
  • Protein Name
    Tigger transposable element-derived protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIGD1 using anti-TIGD1 antibody (A17411-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGD1 antigen affinity purified polyclonal antibody (Catalog # A17411-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIGD1 at approximately 68-70 kDa. The expected band size for TIGD1 is at 67 kDa.
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