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Western Blot analysis of COLO205 cells using TIGD3 Polyclonal Antibody
Western Blot analysis of COLO205 cells using TIGD3 Polyclonal Antibody
Western Blot analysis of COLO205 cells using TIGD3 Polyclonal Antibody

TIGD3 Antibody

CSB-PA004292
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTIGD3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TIGD3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID220359
  • Target name
    TIGD3
  • Target description
    tigger transposable element derived 3
  • Target synonyms
    tigger transposable element-derived protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6B0B8
  • Protein Name
    Tigger transposable element-derived protein 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIGD3 using anti-TIGD3 antibody (A19473-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGD3 antigen affinity purified polyclonal antibody (Catalog # A19473-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIGD3 at approximately 60 kDa. The expected band size for TIGD3 is at 52 kDa.
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