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ProductsBack/TIGD3 antibody, Internal
WB analysis of PANC1 cells using GTX46140 TIGD3 antibody at 0.2-1microg/ml.
WB analysis of PANC1 cells using GTX46140 TIGD3 antibody at 0.2-1microg/ml.
WB analysis of PANC1 cells using GTX46140 TIGD3 antibody at 0.2-1microg/ml.

TIGD3 antibody, Internal

GTX46140
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetTIGD3
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Overview

  • Supplier
    GeneTex
  • Product Name
    TIGD3 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID220359
  • Target name
    TIGD3
  • Target description
    tigger transposable element derived 3
  • Target synonyms
    tigger transposable element-derived protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6B0B8
  • Protein Name
    Tigger transposable element-derived protein 3
  • Scientific Description
    The protein encoded by this gene belongs to the tigger subfamily of the pogo superfamily of DNA-mediated transposons in humans. These proteins are related to DNA transposons found in fungi and nematodes, and more distantly to the Tc1 and mariner transposases. They are also very similar to the major mammalian centromere protein B. The exact function of this gene is not known. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIGD3 using anti-TIGD3 antibody (A19473-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIGD3 antigen affinity purified polyclonal antibody (Catalog # A19473-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIGD3 at approximately 60 kDa. The expected band size for TIGD3 is at 52 kDa.
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