![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639114/GTX639114_T-45194_20231110_WB_B_23111422_862.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
TIGIT antibody [HL2655]
GTX639114
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTIGIT
Overview
- SupplierGeneTex
- Product NameTIGIT antibody [HL2655]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2655
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID201633
- Target nameTIGIT
- Target descriptionT cell immunoreceptor with Ig and ITIM domains
- Target synonymsVSIG9, VSTM3, WUCAM, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain containing 9, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain containing 3, V-set and transmembrane domain-containing protein 3, VSIG9, VSTM3, Washington University cell adhesion molecule
- HostRabbit
- IsotypeIgG
- Protein IDQ495A1
- Protein NameT-cell immunoreceptor with Ig and ITIM domains
- Scientific DescriptionThis gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![MJ whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. MJ whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639114/GTX639114_45271_20231229_WB_Fraction_24010223_942.webp)
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639114/GTX639114_45271_20240105_WB_competitor_watermark_24010821_247.webp)
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639114/GTX639114_45271_20240105_WB_TPM_watermark_24010821_645.webp)




![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2657] (GTX639116) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639116/GTX639116_T-45194_20231027_WB_TPM_watermark_23103019_943.webp)
![IHC-P analysis of human mucosa from appendix tissue using GTX639946 TIGIT antibody [HMV322] HistoMAX?. Distinct TIGIT staining of a subset of lymphocytes.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639946/GTX639946_20240408_IHC-P_2_24040722_213.webp)
![IHC-P analysis of human B-cell chronic lymphocytic leukemia (B-CLL) tissue using GTX639947 TIGIT antibody [HMV323] HistoMAX?. B-CLL with moderate to strong TIGIT positivity of a subset of tumor infiltrating lymphocytes.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639947/GTX639947_20240906_IHC-P_24090601_534.webp)

