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FACS analysis of 2% PFA fixed U2OS cells using GTX04870 TIM-3 antibody [1827CT151.74.68]. Green line : Primary antibody Blue line : Isotype control Dilution : 1:25
FACS analysis of 2% PFA fixed U2OS cells using GTX04870 TIM-3 antibody [1827CT151.74.68]. Green line : Primary antibody Blue line : Isotype control Dilution : 1:25
FACS analysis of 2% PFA fixed U2OS cells using GTX04870 TIM-3 antibody [1827CT151.74.68]. Green line : Primary antibody Blue line : Isotype control Dilution : 1:25

TIM-3 antibody [1827CT151.74.68]

GTX04870
GeneTex
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetHAVCR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TIM-3 antibody [1827CT151.74.68]
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:4000. FACS: 1:25. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1827CT151.74.68
  • Conjugate
    Unconjugated
  • Gene ID84868
  • Target name
    HAVCR2
  • Target description
    hepatitis A virus cellular receptor 2
  • Target synonyms
    CD366; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; Tim-3; TIMD3; TIMD-3
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ8TDQ0
  • Protein Name
    Hepatitis A virus cellular receptor 2
  • Scientific Description
    The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIM 3 using anti-TIM 3 antibody (A00657-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3 antigen affinity purified polyclonal antibody (Catalog # A00657-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3 at approximately 45-70KD. The expected band size for TIM 3 is at 33KD.
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