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ProductsBack/Goat anti-TIM3 / HAVCR2

Goat anti-TIM3 / HAVCR2

EB07113
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetHAVCR2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TIM3 / HAVCR2 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of HepG2 cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID84868
  • Target name
    HAVCR2
  • Target description
    hepatitis A virus cellular receptor 2
  • Target synonyms
    CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3, hepatitis A virus cellular receptor 2, T cell immunoglobulin mucin 3, T-cell immunoglobulin and mucin domain 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, kidney injury molecule-3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_116171.3. Purification: Antigen affinity purified. Names and symbols: HAVCR2; TIM3; hepatitis A virus cellular receptor 2; FLJ14428; KIM-3; TIMD3; Tim-3; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; kidney injury molecule-3
  • Reactivity Supplier
    Human, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIM 3 using anti-TIM 3 antibody (A00657-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3 antigen affinity purified polyclonal antibody (Catalog # A00657-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3 at approximately 45-70KD. The expected band size for TIM 3 is at 33KD.
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