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ICC/IF analysis of Jurkat cells using GTX31299 TIM-4 antibody. Working concentration : 10 microg/ml
ICC/IF analysis of Jurkat cells using GTX31299 TIM-4 antibody. Working concentration : 10 microg/ml
ICC/IF analysis of Jurkat cells using GTX31299 TIM-4 antibody. Working concentration : 10 microg/ml

TIM-4 antibody

GTX31299
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTIMD4
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Overview

  • Supplier
    GeneTex
  • Product Name
    TIM-4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 2 microg/mL. ICC/IF: 10 microg/mL. IHC-P: 2 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID91937
  • Target name
    TIMD4
  • Target description
    T cell immunoglobulin and mucin domain containing 4
  • Target synonyms
    SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, T-cell membrane protein 4, TIM-4, TIMD-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96H15
  • Protein Name
    T-cell immunoglobulin and mucin domain-containing protein 4
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIM 4/TIMD4 using anti-TIM 4/TIMD4 antibody (A06637-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 4/TIMD4 antigen affinity purified polyclonal antibody (Catalog # A06637-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 4/TIMD4 at approximately 42 kDa. The expected band size for TIM 4/TIMD4 is at 42 kDa.
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