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L1 and L2 rat lung lysates probed with Anti TIMP-2 Polyclonal Antibody, Unconjugated (bs-0416R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 24kD. Observed band size: 21kD
L1 and L2 rat lung lysates probed with Anti TIMP-2 Polyclonal Antibody, Unconjugated (bs-0416R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 24kD. Observed band size: 21kD
L1 and L2 rat lung lysates probed with Anti TIMP-2 Polyclonal Antibody, Unconjugated (bs-0416R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 24kD. Observed band size: 21kD

TIMP-2 Polyclonal Antibody

BS-0416R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTIMP2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    TIMP-2 Polyclonal Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7077
  • Target name
    TIMP2
  • Target description
    TIMP metallopeptidase inhibitor 2
  • Target synonyms
    CSC-21K; DDC8; metalloproteinase inhibitor 2; testicular secretory protein Li 57; tissue inhibitor of metalloproteinases 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16035
  • Protein Name
    Metalloproteinase inhibitor 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Tort S et al., 2020 Feb, Burns
    Read more
  • Effects of uterus derived mesenchymal stem cells and their exosomes on ashermans syndrome. Saribas GS et al., 2020 Jan, Acta Histochem
    Read more
  • Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Varghese S et al., 2019 Aug, Int Immunopharmacol
    Read more
  • The inhibitory effect of anti- tumor polysaccharide from Punica granatum on metastasis. Varghese S et al., 2017 Oct, Int J Biol Macromol
    Read more
  • Renoprotective effects of berberine through regulation of the MMPs/TIMPs system in streptozocin-induced diabetic nephropathy in rats. Ni WJ et al., 2015 Oct 5, Eur J Pharmacol
    Read more
  • Single minded 2-s (SIM2-s) gene is expressed in human GBM cells and involved in GBM invasion. He Q et al., 2010 Mar 15, Cancer Biol Ther
    Read more

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Figure 1. Western blot analysis of TIMP2 using anti-TIMP2 antibody (PB9830). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Spleen Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Mouse Thymus Tissue Lysate at 50ug, Lane 5: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMP2 antigen affinity purified polyclonal antibody (Catalog # PB9830) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMP2 at approximately 24 kDa. The expected band size for TIMP2 is at 24 kDa.
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