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Western Blot analysis of SH-SY5Y cells using TIMP-1 Polyclonal Antibody
Western Blot analysis of SH-SY5Y cells using TIMP-1 Polyclonal Antibody
Western Blot analysis of SH-SY5Y cells using TIMP-1 Polyclonal Antibody

TIMP1 Antibody

CSB-PA004293
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTIMP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TIMP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7076
  • Target name
    TIMP1
  • Target description
    TIMP metallopeptidase inhibitor 1
  • Target synonyms
    CLGI, EPA, EPO, HCI, TIMP, TIMP-1, metalloproteinase inhibitor 1, collagenase inhibitor, epididymis secretory sperm binding protein, erythroid potentiating activity, fibroblast collagenase inhibitor, tissue inhibitor of metalloproteinases 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01033
  • Protein Name
    Metalloproteinase inhibitor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Timp-1 using anti-Timp-1 antibody (A00561-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SK-OV-3 whole cell lysates, Lane 2: human 22RV1 whole cell lysates, Lane 3: rat ovarian tissue lysates, Lane 4: mouse ovarian tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Timp-1 antigen affinity purified polyclonal antibody (Catalog # A00561-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Timp-1 at approximately 26KD. The expected band size for Timp-1 is at 23KD.
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