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FACS analysis of CACO-2 cells using GTX03355 TJP2 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Antibody amount : 1microg/1x10? cells for 30 min at 20oC
FACS analysis of CACO-2 cells using GTX03355 TJP2 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Antibody amount : 1microg/1x10? cells for 30 min at 20oC
FACS analysis of CACO-2 cells using GTX03355 TJP2 antibody. Blue : Primary antibody Green : Isotype control Red : Unlabelled sample Antibody amount : 1microg/1x10? cells for 30 min at 20oC

ZO-2 antibody

GTX03355
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTJP2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TJP2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.25-0.5microg/ml. ICC/IF: 5microg/ml. IHC-P: 2-5microg/ml. FACS: 1-3microg/1x106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9414
  • Target name
    TJP2
  • Target description
    tight junction protein 2
  • Target synonyms
    C9DUPq21.11; DFNA51; DUP9q21.11; FHCA1; Friedreich ataxia region gene X104 (tight junction protein ZO-2); PFIC4; tight junction protein ZO-2; X104; ZO2; zona occludens 2; zonula occludens protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UDY2
  • Protein Name
    Tight junction protein ZO-2
  • Scientific Description
    This gene encodes a zonula occluden that is a member of the membrane-associated guanylate kinase homolog family. The encoded protein functions as a component of the tight junction barrier in epithelial and endothelial cells and is necessary for proper assembly of tight junctions. Mutations in this gene have been identified in patients with hypercholanemia, and genomic duplication of a 270 kb region including this gene causes autosomal dominant deafness-51. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TJP2/ZO2 using anti-TJP2/ZO2 antibody (A02774-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP2/ZO2 antigen affinity purified polyclonal antibody (Catalog # A02774-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP2/ZO2 at approximately 150KD. The expected band size for TJP2/ZO2 is at 150KD.
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