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ProductsBack/TKT / Transketolase Antibody (N-Terminus)

TKT / Transketolase Antibody (N-Terminus)

LS-C81255
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTKT
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TKT / Transketolase Antibody (N-Terminus)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7086
  • Target name
    TKT
  • Target description
    transketolase
  • Target synonyms
    epididymis luminal protein 107; epididymis secretory protein Li 48; HEL107; HEL-S-48; SDDHD; TK; TKT1; transketolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Transketolase/TKT using anti-Transketolase/TKT antibody (A02197-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HT1080 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Raji whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat RH35 whole cell lysates, Lane 11: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Transketolase/TKT antigen affinity purified polyclonal antibody (Catalog # A02197-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Transketolase/TKT at approximately 68KD. The expected band size for Transketolase/TKT is at 68KD.
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