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ProductsBack/TLR2 Antibody - C-terminal region (ARP30551_P050)

TLR2 Antibody - C-terminal region (ARP30551_P050)

ARP30551_P050
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Goat, Human, Mouse, Rat, Sheep, Zebra Fish
TargetTLR2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    TLR2 Antibody - C-terminal region (ARP30551_P050)
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID7097
  • Target name
    TLR2
  • Target description
    toll like receptor 2
  • Target synonyms
    CD282, TIL4, toll-like receptor 2, toll/interleukin-1 receptor-like protein 4
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Goat, Human, Mouse, Rat, Sheep, Zebra Fish
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TLR2 using anti-TLR2 antibody (A00131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 (-LPS) whole cell lysates, Lane 2: human THP-1 (+LPS) whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR2 antigen affinity purified polyclonal antibody (A00131) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR2 at approximately 105 kDa. The expected band size for TLR2 is at 90 kDa.
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