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TLR4 Antibody (clone HTA125, Biotin)

LS-C140003
LifeSpan BioSciences
ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetTLR4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TLR4 Antibody (clone HTA125, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. Flo, Func, IF, IP, WB For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HTA125
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID7099
  • Target name
    TLR4
  • Target description
    toll like receptor 4
  • Target synonyms
    ARMD10; CD284; homolog of Drosophila toll; hToll; TLR-4; TOLL; toll like receptor 4 protein; toll-like receptor 4
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TLR4 using anti-TLR4 antibody (A00017-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR4 antigen affinity purified polyclonal antibody (Catalog # A00017-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR4 at approximately 100 kDa. The expected band size for TLR4 is at 96 kDa.
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