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The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA138308(TLR7 Antibody) at dilution 1/10. (Original magnification: 200)
The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA138308(TLR7 Antibody) at dilution 1/10. (Original magnification: 200)
The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using CSB-PA138308(TLR7 Antibody) at dilution 1/10. (Original magnification: 200)

TLR7 Antibody

CSB-PA138308
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTLR7
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Overview

  • Supplier
    Cusabio
  • Product Name
    TLR7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51284
  • Target name
    TLR7
  • Target description
    toll like receptor 7
  • Target synonyms
    IMD74, SLEB17, TLR7-like, toll-like receptor 7, toll-like receptor 7-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NYK1
  • Protein Name
    Toll-like receptor 7
  • Scientific Description
    The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.
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