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Immunohistochemistry analysis of paraffin-embedded human tonsil using TLR9 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded human tonsil using TLR9 Polyclonal Antibody at dilution of 1:200.
Immunohistochemistry analysis of paraffin-embedded human tonsil using TLR9 Polyclonal Antibody at dilution of 1:200.

TLR9 Polyclonal Antibody

E-AB-70125
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TLR9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TLR9 using anti-TLR9 antibody (A00198-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR9 antigen affinity purified polyclonal antibody (Catalog # A00198-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR9 at approximately 116 kDa. The expected band size for TLR9 is at 116 kDa.
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