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ProductsBack/TMEM30B Antibody
Western Blot analysis of K562 cells using Cdc50B Polyclonal Antibody
Western Blot analysis of K562 cells using Cdc50B Polyclonal Antibody
Western Blot analysis of K562 cells using Cdc50B Polyclonal Antibody

TMEM30B Antibody

CSB-PA007647
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetTMEM30B
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Overview

  • Supplier
    Cusabio
  • Product Name
    TMEM30B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID161291
  • Target name
    TMEM30B
  • Target description
    transmembrane protein 30B
  • Target synonyms
    CDC50B; cell cycle control protein 50B; P4-ATPase flippase complex beta subunit TMEM30B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3MIR4
  • Protein Name
    Cell cycle control protein 50B
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMEM30B using anti-TMEM30B antibody (A13764-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM30B antigen affinity purified polyclonal antibody (Catalog # A13764-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM30B at approximately 39 kDa. The expected band size for TMEM30B is at 39 kDa.
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