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Transmembrane Protein 30B Antibody (YA2220, Rabbit)

HY-P82475
MedChem Express
TargetTMEM30B
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Transmembrane Protein 30B Antibody (YA2220, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID161291
  • Target name
    TMEM30B
  • Target description
    transmembrane protein 30B
  • Target synonyms
    CDC50B, cell cycle control protein 50B, P4-ATPase flippase complex beta subunit TMEM30B
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Transmembrane Protein 30B Antibody (YA2220) is a non-conjugated IgG antibody, targeting Transmembrane Protein 30B, with a predicted molecular weight of 39 kDa (observed band size: 39 kDa). Transmembrane Protein 30B Antibody (YA2220) can be used for WB experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMEM30B using anti-TMEM30B antibody (A13764-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM30B antigen affinity purified polyclonal antibody (Catalog # A13764-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM30B at approximately 39 kDa. The expected band size for TMEM30B is at 39 kDa.
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