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ProductsBack/TMPRSS2 Antibody - N-terminal region (OAAB04388)

TMPRSS2 Antibody - N-terminal region (OAAB04388)

OAAB04388
Aviva Systems Biology
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTMPRSS2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    TMPRSS2 Antibody - N-terminal region (OAAB04388)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Format
    Liquid PBS with 0.09% (W/V) sodium azide.
  • Gene ID7113
  • Target name
    TMPRSS2
  • Target description
    transmembrane serine protease 2
  • Target synonyms
    PRSS10, transmembrane protease serine 2, epitheliasin, serine protease 10, transmembrane protease, serine 2
  • Host
    Rabbit
  • Isotype
    IgG
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMPRSS2 using anti-TMPRSS2 antibody (M00666). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS2 antigen affinity purified monoclonal antibody (Catalog # M00666) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS2 at approximately 54 kDa. The expected band size for TMPRSS2 is at 54 kDa.
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