TNF alpha antibody [CH8820]

GeneTex

TNF alpha antibody [CH8820]

9

This monoclonal antibody recognizes both recombinant and native human TNF-alpha.

ELISA: Use at an assay dependent dilution. GTX18696 can be used as a capture antibody in sandwich ELISA applications for human TNF-alpha detection in combination with a monoclonal tracer/detection antibody (biotinconjugated). 4 pg/ml of TNF-alpha in serum/plasma or medium can be detected with an assay range of 0 to 2000 pg/ml. Suggested capture coating dose: 1.0-2.0microg/well IHC: Use at a concentration of 10 microg/ml. Reacts with formalin-fixed paraffin-embedded tissues at a monoclonal antibody concentration of 10microg/mL. Strong reaction with normal kidney, liver with cirrhosis, and normal lung samples, positive reaction with lung cancer samples.Neut: GTX18696 neutralizes TNF-alpha activity in vitro. Use 2-5 ng of GTX18696 per microg of MAb. Optimal dilutions/concentrations should be determined by the end user.

ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

CH8820

1 mg/ml

Unconjugated

Liquid

TNF

tumor necrosis factor

APC1 protein; DIF; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNF-alpha; TNFSF2; TNLG1F; tumor necrosis factor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

Mouse

IgG1

Tumor necrosis factor

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Human

-20°C or -80°C,2°C to 8°C

12352203