Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [2C8] (GTX28348) (1 microg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.
Indirect ELISA analysis was performed by coating the plate with recombinant 293T expressed, human Fc- tagged TNF alpha(54-233 a.a.) (201.71-3.15 nM). Coated protein was probed with TNF alpha antibody [2C8] (GTX28348) (1 microg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.
TNF alpha antibody [2C8]
GTX28348
ApplicationsELISA, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetTNF
Overview
- SupplierGeneTex
- Product NameTNF alpha antibody [2C8]
- Delivery Days Customer9
- Antibody SpecificityThis antibody is effective in inhibition of the biological activity of recombinant and natural TNF-α.
- Application Supplier Note*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsELISA, ImmunoHistoChemistry, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2C8
- ConjugateUnconjugated
- Gene ID7124
- Target nameTNF
- Target descriptiontumor necrosis factor
- Target synonymsAPC1 protein; DIF; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNF-alpha; TNFSF2; TNLG1F; tumor necrosis factor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
- HostMouse
- IsotypeIgG1
- Protein IDP01375
- Protein NameTumor necrosis factor
- Scientific DescriptionThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction2°C to 8°C
- UNSPSC12352203