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TNF Receptor Associated Factor 6 (TRAF6) Polyclonal Antibody

CAU22170
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetTraf6
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Overview

  • Supplier
    Biomatik
  • Product Name
    TNF Receptor Associated Factor 6 (TRAF6) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22034
  • Target name
    Traf6
  • Target description
    TNF receptor-associated factor 6
  • Target synonyms
    2310003F17Rik, C630032O20Rik, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, RING-type E3 ubiquitin transferase TRAF6
  • Host
    Rabbit
  • Protein IDP70196
  • Protein Name
    TNF receptor-associated factor 6
  • Scientific Description
    The TNF Receptor Associated Factor 6 (TRAF6) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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TargetTraf6
  • SizePrice
Figure 1. Western blot analysis of TRAF6 using anti-TRAF6 antibody (A00185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6 antigen affinity purified polyclonal antibody (Catalog # A00185) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6 at approximately 60-65KD. The expected band size for TRAF6 is at 60KD.
Product group Antibodies
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Anti-TRAF6 Antibody Picoband(r)A00185-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetTraf6
  • SizePrice