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Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti-TRAF6 Polyclonal Antibody, Unconjugated (bs-1184R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti-TRAF6 Polyclonal Antibody, Unconjugated (bs-1184R) followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human oral squamous cell carcinoma labeled with Anti-TRAF6 Polyclonal Antibody, Unconjugated (bs-1184R) followed by conjugation to the secondary antibody and DAB staining

TRAF6 Polyclonal Antibody

BS-1184R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetTraf6
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Overview

  • Supplier
    Bioss
  • Product Name
    TRAF6 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID22034
  • Target name
    Traf6
  • Target description
    TNF receptor-associated factor 6
  • Target synonyms
    2310003F17Rik, C630032O20Rik, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, RING-type E3 ubiquitin transferase TRAF6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP70196
  • Protein Name
    TNF receptor-associated factor 6
  • Reactivity
    Bovine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wu Z, Ding L, Bao J, et al. Co-infection of Mycoplasma gallisepticum and Escherichia coli Triggers Inflammatory Injury Involving the IL-17 Signaling Pathway. Front Microbiol. 2019,10:2615. doi: 10.3389/fmicb.2019.02615
    Read this paper
  • Li C, Yu Y, Li W, et al. Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway. Sci Rep. 2017,7(1):5843. doi: 10.1038/s41598-017-06021-5
    Read this paper

Datasheet

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TargetTraf6
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Figure 1. Western blot analysis of TRAF6 using anti-TRAF6 antibody (A00185). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 whole cell lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6 antigen affinity purified polyclonal antibody (Catalog # A00185) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6 at approximately 60-65KD. The expected band size for TRAF6 is at 60KD.
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TargetTraf6
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