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ProductsBack/TNF Receptor II antibody [6F13]

TNF Receptor II antibody [6F13]

GTX53103
GeneTex
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetTnfrsf1b
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Overview

  • Supplier
    GeneTex
  • Product Name
    TNF Receptor II antibody [6F13]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6F13
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID21938
  • Target name
    Tnfrsf1b
  • Target description
    tumor necrosis factor receptor superfamily, member 1b
  • Target synonyms
    CD120b, TNF-R-II, TNF-R2, TNF-R75, TNF-alphaR2, TNFBR, TNFR80, TNFRII, TNFalpha-R2, Tnfr-1, Tnfr2, p75, tumor necrosis factor receptor superfamily member 1B, TNF receptor beta chain, TNF-RII, TNFR-II, p75 TNFR, p80 TNF-alpha receptor, tumor necrosis factor receptor 2, tumor necrosis factor receptor type II
  • Host
    Hamster
  • Isotype
    IgG
  • Protein IDP25119
  • Protein Name
    Tumor necrosis factor receptor superfamily member 1B
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II antigen affinity purified polyclonal antibody (Catalog # PB9515) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II at approximately 75 kDa. The expected band size for TNF Receptor II is at 75 kDa.
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