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ProductsBack/TNFRSF10B / Killer / DR5 Antibody

TNFRSF10B / Killer / DR5 Antibody

LS-C812941
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTNFRSF10B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TNFRSF10B / Killer / DR5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8795
  • Target name
    TNFRSF10B
  • Target description
    TNF receptor superfamily member 10b
  • Target synonyms
    CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, tumor necrosis factor receptor superfamily member 10B, Fas-like protein, TNF-related apoptosis-inducing ligand receptor 2, apoptosis inducing protein TRICK2A/2B, apoptosis inducing receptor TRAIL-R2, cytotoxic TRAIL receptor-2, death domain containing receptor for TRAIL/Apo-2L, death receptor 5, p53-regulated DNA damage-inducible cell death receptor(killer), tumor necrosis factor receptor superfamily, member 10b, tumor necrosis factor receptor-like protein ZTNFR9
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DR5/TNFRSF10B using anti-DR5/TNFRSF10B antibody (A00410-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR5/TNFRSF10B antigen affinity purified polyclonal antibody (Catalog # A00410-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR5/TNFRSF10B at approximately 43KD,48KD. The expected band size for DR5/TNFRSF10B is at 48KD.
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