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Western Blot analysis of 293 cells using OPG Polyclonal Antibody
Western Blot analysis of 293 cells using OPG Polyclonal Antibody
Western Blot analysis of 293 cells using OPG Polyclonal Antibody

TNFRSF11B Antibody

CSB-PA003597
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTNFRSF11B
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Overview

  • Supplier
    Cusabio
  • Product Name
    TNFRSF11B Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4982
  • Target name
    TNFRSF11B
  • Target description
    TNF receptor superfamily member 11b
  • Target synonyms
    OCIF; OPG; osteoclastogenesis inhibitory factor; osteoprotegerin; PDB5; TR1; tumor necrosis factor receptor superfamily member 11B; tumor necrosis factor receptor superfamily, member 11b
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00300
  • Protein Name
    Tumor necrosis factor receptor superfamily member 11B
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNFRSF11B using anti-TNFRSF11B antibody (A00863). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF11B antigen affinity purified polyclonal antibody (Catalog # A00863) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF11B at approximately 55 kDa. The expected band size for TNFRSF11B is at 46 kDa.
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Immunofluorescent staining of human cell line BJ [Human fibroblast] shows localization to the Golgi apparatus.
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