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ProductsBack/TNFRSF1B / TNFR2 Antibody (Asp335)

TNFRSF1B / TNFR2 Antibody (Asp335)

LS-C40001
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNFRSF1B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TNFRSF1B / TNFR2 Antibody (Asp335)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot and Immunoprecipitation. Western Blot 1:1000. Immunoprecipitation 1:100.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP (1:100), WB (1:1000) Suitable for use in Western Blot and Immunoprecipitation. Western Blot 1:1000. Immunoprecipitation 1:100.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7133
  • Target name
    TNFRSF1B
  • Target description
    TNF receptor superfamily member 1B
  • Target synonyms
    CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR, tumor necrosis factor receptor superfamily member 1B, TNF-R2, TNF-RII, p75 TNF receptor, p80 TNF-alpha receptor, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, tumor necrosis factor receptor 2, tumor necrosis factor receptor type II
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNF Receptor II/TNFRSF1B using anti-TNF Receptor II/TNFRSF1B antibody (A01437-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II/TNFRSF1B antigen affinity purified polyclonal antibody (Catalog # A01437-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II/TNFRSF1B at approximately 75 kDa. The expected band size for TNF Receptor II/TNFRSF1B is at 75 kDa.
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