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TNFSF12 Antibody

CAB5659
Assay Genie
TargetTNFSF12
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    TNFSF12 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8742
  • Target name
    TNFSF12
  • Target description
    TNF superfamily member 12
  • Target synonyms
    APO3 ligand; APO3/DR3 ligand; APO3L; DR3LG; TNF-related WEAK inducer of apoptosis; TNLG4A; tumor necrosis factor (ligand) superfamily, member 12; tumor necrosis factor ligand 4A; tumor necrosis factor ligand superfamily member 12; tumor necrosis factor superfamily member 12; TWEAK
  • Host
    Rabbit
  • Protein IDO43508
  • Protein Name
    Tumor necrosis factor ligand superfamily member 12
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TWEAK using anti-TWEAK antibody (A02009-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MCF-7 cell lysate, Lane 2: human PANC-1 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWEAK antigen affinity purified polyclonal antibody (Catalog # A02009-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWEAK at approximately 27KD. The expected band size for TWEAK is at 27KD.
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