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Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA023998LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA023998LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA023998LA01HU at dilution of 1:100

TNIK Antibody

CSB-PA023998LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNIK
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Overview

  • Supplier
    Cusabio
  • Product Name
    TNIK Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23043
  • Target name
    TNIK
  • Target description
    TRAF2 and NCK interacting kinase
  • Target synonyms
    MRT54; TRAF2 and NCK-interacting protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKE5
  • Protein Name
    TRAF2 and NCK-interacting protein kinase
  • Scientific Description
    Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNIK using anti-TNIK antibody (A03934-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNIK antigen affinity purified polyclonal antibody (Catalog # A03934-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNIK at approximately 180,150 kDa. The expected band size for TNIK is at 155 kDa.
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