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TNIK antibody

GTX124264
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetTNIK
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Overview

  • Supplier
    GeneTex
  • Product Name
    TNIK antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23043
  • Target name
    TNIK
  • Target description
    TRAF2 and NCK interacting kinase
  • Target synonyms
    MRT54; TRAF2 and NCK-interacting protein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKE5
  • Protein Name
    TRAF2 and NCK-interacting protein kinase
  • Scientific Description
    Germinal center kinases (GCKs), such as TNIK, are characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function (Fu et al., 1999 [PubMed 10521462]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNIK using anti-TNIK antibody (A03934-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNIK antigen affinity purified polyclonal antibody (Catalog # A03934-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNIK at approximately 180,150 kDa. The expected band size for TNIK is at 155 kDa.
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