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TNS3 Antibody (OAAF02499)

OAAF02499
Aviva Systems Biology
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
TargetTNS3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    TNS3 Antibody (OAAF02499)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Liquid
  • Gene ID64759
  • Target name
    TNS3
  • Target description
    tensin 3
  • Target synonyms
    TEM6; TENS1; tensin-3; tensin-like SH2 domain containing 1; tensin-like SH2 domain-containing protein 1; thyroid specific PTB domain protein; tumor endothelial marker 6
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNS3 using anti-TNS3 antibody (A07345-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNS3 antigen affinity purified polyclonal antibody (Catalog # A07345-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNS3 at approximately 200 kDa. The expected band size for TNS3 is at 155 kDa.
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