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TOMM20 Antibody (30-110 aa, Internal)

LS-C386458
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTOMM20
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TOMM20 Antibody (30-110 aa, Internal)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:40000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9804
  • Target name
    TOMM20
  • Target description
    translocase of outer mitochondrial membrane 20
  • Target synonyms
    MAS20, MOM19, TOM20, mitochondrial import receptor subunit TOM20 homolog, mitochondrial 20 kDa outer membrane protein, outer mitochondrial membrane receptor Tom20, translocase of outer mitochondrial membrane 20 homolog type II
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOMM20 using anti-TOMM20 antibody (A04039-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM20 antigen affinity purified polyclonal antibody (Catalog # A04039-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM20 at approximately 16 kDa. The expected band size for TOMM20 is at 16 kDa.
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