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Western blot All lanes: TOMM70A antibody at 6microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: TOMM70A antibody at 6microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: TOMM70A antibody at 6microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa

TOMM70 Antibody

CSB-PA024055ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTOMM70
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOMM70 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9868
  • Target name
    TOMM70
  • Target description
    translocase of outer mitochondrial membrane 70
  • Target synonyms
    TOMM70A, Tom70, mitochondrial import receptor subunit TOM70, mitochondrial precursor proteins import receptor, translocase of outer membrane 70 kDa subunit, translocase of outer mitochondrial membrane 70 homolog A, translocase of outer mitochondrial membrane protein 70
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94826
  • Protein Name
    Mitochondrial import receptor subunit TOM70
  • Scientific Description
    Receptor that accelerates the import of all mitochondrial precursor proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM70 antigen affinity purified polyclonal antibody (Catalog # A09172-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM70 at approximately 67 kDa. The expected band size for TOMM70 is at 67 kDa.
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