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Western blot All lanes: TOMM70A antibody at 6microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: TOMM70A antibody at 6microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: TOMM70A antibody at 6microg/ml + MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa

TOMM70 Antibody

CSB-PA024055ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTOMM70
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOMM70 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9868
  • Target name
    TOMM70
  • Target description
    translocase of outer mitochondrial membrane 70
  • Target synonyms
    mitochondrial import receptor subunit TOM70; mitochondrial precursor proteins import receptor; Tom70; TOMM70A; translocase of outer membrane 70 kDa subunit; translocase of outer mitochondrial membrane 70 homolog A; translocase of outer mitochondrial membrane protein 70
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94826
  • Protein Name
    Mitochondrial import receptor subunit TOM70
  • Scientific Description
    Receptor that accelerates the import of all mitochondrial precursor proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM70 antigen affinity purified polyclonal antibody (Catalog # A09172-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM70 at approximately 67 kDa. The expected band size for TOMM70 is at 67 kDa.
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