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TOMM70A Antibody

LS-C408767
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTOMM70
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TOMM70A Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 67kDa, while the observed MW by Western blot was 75kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 67kDa, while the observed MW by Western blot was 75kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9868
  • Target name
    TOMM70
  • Target description
    translocase of outer mitochondrial membrane 70
  • Target synonyms
    TOMM70A, Tom70, mitochondrial import receptor subunit TOM70, mitochondrial precursor proteins import receptor, translocase of outer membrane 70 kDa subunit, translocase of outer mitochondrial membrane 70 homolog A, translocase of outer mitochondrial membrane protein 70
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOMM70 using anti-TOMM70 antibody (A09172-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOMM70 antigen affinity purified polyclonal antibody (Catalog # A09172-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOMM70 at approximately 67 kDa. The expected band size for TOMM70 is at 67 kDa.
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