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Western Blot analysis of COS7 cells using Topo IIalpha Polyclonal Antibody
Western Blot analysis of COS7 cells using Topo IIalpha Polyclonal Antibody
Western Blot analysis of COS7 cells using Topo IIalpha Polyclonal Antibody

TOP2A Antibody

CSB-PA004317
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey
TargetTOP2A
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOP2A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7153
  • Target name
    TOP2A
  • Target description
    DNA topoisomerase II alpha
  • Target synonyms
    DNA gyrase; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; TOP2; TOP2alpha; TOPIIA; topoisomerase (DNA) II alpha 170kDa; TP2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11388
  • Protein Name
    DNA topoisomerase 2-alpha
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TOP2A using anti-TOP2A antibody (A00953). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOP2A antigen affinity purified polyclonal antibody (Catalog # A00953) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOP2A at approximately 174KD. The expected band size for TOP2A is at 174KD.
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Anti-Topoisomerase II alpha/TOP2A Antibody Picoband(r)A00953-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTOP2A
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Product group Antibodies
References
GeneTex
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TargetTOP2A
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