Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa. Lane 2: HepG2. Lane 3: HEK-293. Lane 4: SH-SY5Y. Lane 5: MDCK. Lane 6: PC12. Lane 7: CMT-93. Lane 8: Neuro 2A. Lane 9: 3T3.
Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa. Lane 2: HepG2. Lane 3: HEK-293. Lane 4: SH-SY5Y. Lane 5: MDCK. Lane 6: PC12. Lane 7: CMT-93. Lane 8: Neuro 2A. Lane 9: 3T3.
Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: 23B11]
AM00154PU-N
Overview
- SupplierOriGene
- Product NameTopoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: 23B11]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID23B11
- Gene ID7150
- Target nameTOP1
- Target descriptionDNA topoisomerase I
- Target synonymsDNA topoisomerase 1; Scl-70 antigen; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase
- HostMouse
- IsotypeIgG1
- Scientific DescriptionTopoisomerase I (TOP1) mouse monoclonal antibody, clone 23B11, Purified
- ReactivityHuman
- UNSPSC12352203