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Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa. Lane 2: HepG2. Lane 3: HEK-293. Lane 4: SH-SY5Y. Lane 5: MDCK. Lane 6: PC12. Lane 7: CMT-93. Lane 8: Neuro 2A. Lane 9: 3T3.
Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa. Lane 2: HepG2. Lane 3: HEK-293. Lane 4: SH-SY5Y. Lane 5: MDCK. Lane 6: PC12. Lane 7: CMT-93. Lane 8: Neuro 2A. Lane 9: 3T3.
Detection of endogenous Topoisomerase I: whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with TOP1 / SCL-70 antibody Cat.-No. AM00154PU-N (0.5 ug/ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa. Lane 2: HepG2. Lane 3: HEK-293. Lane 4: SH-SY5Y. Lane 5: MDCK. Lane 6: PC12. Lane 7: CMT-93. Lane 8: Neuro 2A. Lane 9: 3T3.

Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: 23B11]

Research Use Only
AM00154PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTOP1
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Topoisomerase I (TOP1) Mouse Monoclonal Antibody [Clone ID: 23B11]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    23B11
  • Gene ID7150
  • Target name
    TOP1
  • Target description
    DNA topoisomerase I
  • Target synonyms
    DNA topoisomerase 1; Scl-70 antigen; TOPI; topoisomerase (DNA) I; type I DNA topoisomerase
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Topoisomerase I (TOP1) mouse monoclonal antibody, clone 23B11, Purified
  • Reactivity
    Human
  • UNSPSC
    12352203