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Western Blot analysis of HeLa cells using LAP1B Polyclonal Antibody
Western Blot analysis of HeLa cells using LAP1B Polyclonal Antibody
Western Blot analysis of HeLa cells using LAP1B Polyclonal Antibody

TOR1AIP1 Antibody

CSB-PA003136
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTOR1AIP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOR1AIP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26092
  • Target name
    TOR1AIP1
  • Target description
    torsin 1A interacting protein 1
  • Target synonyms
    LAP1, LAP1B, LAP1C, LGMD2Y, torsin-1A-interacting protein 1, lamin-associated protein 1B, lamina-associated polypeptide 1B, torsin A interacting protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5JTV8
  • Protein Name
    Torsin-1A-interacting protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TOR1AIP1 using anti-TOR1AIP1 antibody (A06631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOR1AIP1 antigen affinity purified polyclonal antibody (Catalog # A06631-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOR1AIP1 at approximately 66 kDa. The expected band size for TOR1AIP1 is at 66 kDa.
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Immunohistochemical staining of human cerebral cortex, kidney, lymph node and testis using Anti-TOR1AIP1 antibody HPA047151 (A) shows similar protein distribution across tissues to independent antibody HPA050546 (B).
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