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TOR1AIP1 / LAP1 Antibody

LS-C830806
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTOR1AIP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TOR1AIP1 / LAP1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:300)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID26092
  • Target name
    TOR1AIP1
  • Target description
    torsin 1A interacting protein 1
  • Target synonyms
    LAP1, LAP1B, LAP1C, LGMD2Y, torsin-1A-interacting protein 1, lamin-associated protein 1B, lamina-associated polypeptide 1B, torsin A interacting protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOR1AIP1 using anti-TOR1AIP1 antibody (A06631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOR1AIP1 antigen affinity purified polyclonal antibody (Catalog # A06631-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOR1AIP1 at approximately 66 kDa. The expected band size for TOR1AIP1 is at 66 kDa.
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