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ProductsBack/Phospho-TORC2 (Ser171) Polyclonal Antibody
L1 human rectal carcinoma lysates L2 human gastric carcinoma lysates, probed with Anti Phospho-Torc2/Crtc2(Ser171) Polyclonal Antibody, Unconjugated (bs-3415R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 73kD. Observed band size:73kD.\n
L1 human rectal carcinoma lysates L2 human gastric carcinoma lysates, probed with Anti Phospho-Torc2/Crtc2(Ser171) Polyclonal Antibody, Unconjugated (bs-3415R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 73kD. Observed band size:73kD.\n
L1 human rectal carcinoma lysates L2 human gastric carcinoma lysates, probed with Anti Phospho-Torc2/Crtc2(Ser171) Polyclonal Antibody, Unconjugated (bs-3415R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 73kD. Observed band size:73kD.\n

Phospho-TORC2 (Ser171) Polyclonal Antibody

BS-3415R
Bioss
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityCanine, Human, Mouse, Porcine, Rat
TargetCRTC2
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Overview

  • Supplier
    Bioss
  • Product Name
    Phospho-TORC2 (Ser171) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID200186
  • Target name
    CRTC2
  • Target description
    CREB regulated transcription coactivator 2
  • Target synonyms
    TORC-2, TORC2, CREB-regulated transcription coactivator 2, transducer of regulated cAMP response element-binding protein (CREB) 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53ET0
  • Protein Name
    CREB-regulated transcription coactivator 2
  • Reactivity
    Canine, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Zhou TT, Zhao T, Ma F, et al. Small molecule IVQ, as a prodrug of gluconeogenesis inhibitor QVO, efficiently ameliorates glucose homeostasis in type 2 diabetic mice. Acta Pharmacol Sin. 2019,40(9):1193-1204. doi: 10.1038/s41401-018-0208-2
    Read this paper
  • Seok S, Fu T, Choi SE, et al. Transcriptional regulation of autophagy by an FXR-CREB axis. Nature. 2014,516(7529):108-11. doi: 10.1038/nature13949
    Read this paper
  • Cicerchi C, Li N, Kratzer J, et al. Uric acid-dependent inhibition of AMP kinase induces hepatic glucose production in diabetes and starvation: evolutionary implications of the uricase loss in hominids. FASEB J. 2014,28(8):3339-50. doi: 10.1096/fj.13-243634
    Read this paper

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Figure 1. Western blot analysis of TORC2 using anti-TORC2 antibody (A01118-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TORC2 antigen affinity purified polyclonal antibody (Catalog # A01118-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TORC2 at approximately 73KD. The expected band size for TORC2 is at 73KD.
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