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ProductsBack/TOX Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate All lanes: TOX antibody at 5.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate All lanes: TOX antibody at 5.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, U87 whole cell lysate All lanes: TOX antibody at 5.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa

TOX Antibody

CSB-PA024073LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTOX
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOX Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9760
  • Target name
    TOX
  • Target description
    thymocyte selection associated high mobility group box
  • Target synonyms
    thymocyte selection-associated high mobility group box protein TOX; thymus high mobility group box protein TOX; TOX1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO94900
  • Protein Name
    Thymocyte selection-associated high mobility group box protein TOX
  • Scientific Description
    May play a role in regulating T-cell development.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOX using anti-TOX antibody (A08441-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX antigen affinity purified polyclonal antibody (Catalog # A08441-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOX at approximately 57 kDa. The expected band size for TOX is at 57 kDa.
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