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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA077768(TPM2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA077768(TPM2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA077768(TPM2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

TPM2 Antibody

CSB-PA077768
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTPM2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TPM2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7169
  • Target name
    TPM2
  • Target description
    tropomyosin 2
  • Target synonyms
    AMCD1; DA1; DA2B; DA2B4; epididymis secretory protein Li 273; HEL-S-273; NEM4; nemaline myopathy type 4; TMSB; tropomyosin 2 (beta); tropomyosin beta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07951
  • Protein Name
    Tropomyosin beta chain
  • Scientific Description
    This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. TPM2 has been shown to interact with RRAD, PDLIM7 and TPM1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Muscle lysates probed with TPM2 Polyclonal Antibody, Unconjugated (bs-1243R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat H9C2(2-1) whole cell lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tropomyosin 2/TPM2 antigen affinity purified polyclonal antibody (Catalog # A03082-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tropomyosin 2/TPM2 at approximately 38 kDa. The expected band size for Tropomyosin 2/TPM2 is at 33 kDa.
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