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TPM2 Antibody

LS-C771342
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTPM2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TPM2 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7169
  • Target name
    TPM2
  • Target description
    tropomyosin 2
  • Target synonyms
    AMCD1; DA1; DA2B; DA2B4; epididymis secretory protein Li 273; HEL-S-273; NEM4; nemaline myopathy type 4; TMSB; tropomyosin 2 (beta); tropomyosin beta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tropomyosin 2/TPM2 using anti-Tropomyosin 2/TPM2 antibody (A03082-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat H9C2(2-1) whole cell lysates, Lane 3: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tropomyosin 2/TPM2 antigen affinity purified polyclonal antibody (Catalog # A03082-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tropomyosin 2/TPM2 at approximately 38 kDa. The expected band size for Tropomyosin 2/TPM2 is at 33 kDa.
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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA077768(TPM2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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